The purity, content, total weight and actual weight of the vial lyophilized peptide

- Apr 13, 2018-

Release Date: 2018-01-18 Author: Jiangsu Jitai vial lyophilized peptide Technology Co., Ltd. Hits: 18

Based on the total weight and the purity of the HPLC analysis, how is the target vial lyophilized peptide content determined in the sample? Our company's vial lyophilized peptides are shipped based on total weight, which is the actual weight of the vial freeze-dried peptide after lyophilization, including other impurities such as residual moisture from the opposite charge in the lyophilization process. The weight of the salt and other ions.

The purity of the vial lyophilized peptide refers to the proportion of the target vial lyophilized peptide in the sample at a wavelength of 214 nm. The sample may contain missing sequences, short fragments, incomplete deprotection sequences, and the like. The purity of the vial lyophilized peptides was analyzed by HPLC at the 214 nm maximum absorption peak of the vial lyophilized peptide. The vial lyophilized peptides typically also contained water and salt, but the purity of the vial lyophilized peptides did not count them. .

The vial lyophilized peptide content refers to the percentage of all vial freeze-dried peptides in the sample. The sample contains all the vial lyophilized peptides and moisture, salt, etc. The vial lyophilized peptide content is obtained by amino acid analysis.

The absolute content of the vial lyophilized peptide is the product of the purity of the target vial lyophilized peptide and its content in the sample.

How to preserve vials of lyophilized peptides

Release Date: 2017-10-14 Author: Jiangsu Jitai vial lyophilized peptide Technology Co., Ltd. Hits: 51

The vials of lyophilized peptides are very stable at -20[deg.] C., particularly freeze-dried and stored in a desiccator. Lyophilized vials can be allowed to stand at room temperature before they are exposed to air. This will reduce the effect of humidity, and when freeze-drying is not possible, the best method is to store it in a small sample size.

For vials of lyophilized peptides containing Cys, Met or TrP, the deoxygenation buffer is essential for their solubilization because the vials of lyophilized peptides can easily be air oxidized. Prior to bottle closure, they slowly flow through the vials of lyophilized peptide nitrogen or Argon also reduces oxidation. The vial freeze-dried peptides containing Gln or Asn are also susceptible to degradation and all of these vial lyophilized peptides have a limited life span compared to those vials containing these problematic glycosides.